分别用不同的培养基培养树舌,实验结果表明,合成培养基产糖量最高,树舌胞外多糖的产率为61.75mg/100 mL,胞内多糖的产率为0.51 mg/100 mL。树舌多糖纯化的最优纯化方案为:用1.5 g/50 mL活性炭进行脱色,按V(样品)∶V(三氯甲烷)∶V(正丁醇)=25∶5∶1的比例除蛋白,用1.5倍体积乙醇沉淀多糖。按优化的方案纯化树舌粗多糖,纯化后粗多糖中多糖的含量由21.6%上升为33.6%,多糖损耗率为52.7%。

Ganoderma applanatum is cultured in different mediums.The results show that synthetic medium has the highest sugar yield.The production of Ganoderma applanatum extracellular polysaccharide is 61.75 mg/100 mL and the intracellular polysaccharide is 0.51 mg/100 mL.The amount of activated carbon for decolorization is 1.5 g/50 mL in purifying ganoderma applanatum crude polysaccharide.The ratio of deproteinization processing is sample∶ chloroform∶ butanol=25∶ 5∶ 1;Ethanol of 1.5 volume was used to precipitate the polysaccharide.Ganoderma applanatum crude polysaccharide purification was optimized.After purification,the polysaccharide content in crude polysaccharide is increased from 21.6% to 33.6%,while the polysaccharide is lost 52.7%.