以乙醇为溶剂从脱脂豆粕中提取大豆皂苷,AB-8大孔树脂纯化后对其进行薄层层析、红外光谱和高效液相色谱定性分析,同时研究其抗油脂氧化能力。结果表明:脱脂豆粕提取物经纯化后得到的大豆皂苷具有抗豆油氧化能力,且有剂量效应关系,0.5%大豆皂苷纯化品抗油脂氧化能力较强,相当于0.02%BHT,随着时间的延长,作用甚至强于0.02%BHT;大豆皂苷与柠檬酸、抗坏血酸均有较好的协同作用,并且抗坏血酸的增效作用强于柠檬酸,是较好的天然抗氧化剂。

Defatted soy meals were extracted with ethanol aqueous solution to soybean saponins and the extract was analyzed qualitatively by means of thin-layer chromatography,infraed spectroscopy and high performance liquid chromatography.Meanwhile,its protecting effects against the lipid oxidation in bean oil were investigated.The major components in the defatted soy meals were soybean saponins.The extract could effectively protect bean oil from lipid oxidation in a dose-dependent manner and presented higher protecting effect at the dosage of 0.5%,which,however,corresponded with that of 0.02% BHT.Synergistic protecting effects between ascorbic acid or citric acid.Soybean saponins from defatted soy meals are a potential natural antioxidant.