对马铃薯块茎粗多酚抗氧化活性进行研究。采用乙醇、丙酮、蒸馏水3种溶剂提取马铃薯块茎中粗多酚类物质,通过粗多酚提取液对羟自由基(.OH)、超氧自由基(O2-.)、亚硝酸盐(NO2-)的清除和还原能力测定,比较3种酚提液的体外抗氧化活性,并测定其中总多酚含量。结果显示:3种粗多酚提取液总多酚含量为0.33~0.40 mg/mL;在不同抗氧化体系中,3种酚提液均具有良好的抗氧化活性,抗氧化能力的大小顺序为60%乙醇酚提液>60%丙酮酚提液>蒸馏水酚提液,抗氧化活性的大小与总多酚含量的高低呈正相关。
The antioxidant activities of crude polyphenol extracts from potato tuber were studied.The crude polyphenol extracts were extracted using ethanol,acetone and water.Antioxidant activities of three polyphenol extracts were studied including ·OH free radical scavenging,O-2· free radical scavenging,NO-2 scavenging and reducing power.The contents of polyphenol were also determined.Results showed that total polyphenol contents were 0.33~0.40 mg/mL.Three crude polyphenol extracts exhibit excellent antioxidation activitiy in different antioxidant assays.The order of antioxidation abilities were: 60% ethanol extract>60% acetone extract>water extract.This was positively correlated with the total polyphenol contents of the different polyphenol extracts.