采用雅致放射毛霉转化腺嘌呤合成ATP,系统地研究了菌体通透化方法,考察了它对腺嘌呤转化率、ATP产量的影响,筛选出较佳方法,并采用电导率法验证菌体通透性的改变情况。结果表明,通过干燥方法处理毛霉菌体,在含有3 g/L腺嘌呤的反应液中,转化生成10.13 g/L ATP,腺嘌呤转化率为82.76%,比对照组提高202.39%;干燥处理后的毛霉菌体的电导率显著提高,且电导率随干燥时间的变化趋势与ATP产量随干燥时间的变化趋势基本吻合,表明干燥方法真实地提高了毛霉菌的通透性,进而提高了腺嘌呤转化率及ATP产量。

Methods related to the modification of cell permeability were studied systematically in Actinomucor elegans.The correlation between cell membrane permeability determined by electrical conductivity method and cell enzymatic activity for ATP production from adenine was investigated.As a result,drying method was selected.After optimization by drying method in production process,10.13 g/L ATP was accumulated from 3 g/L adenine and the molar conversion ratio was 82.76%,which increased by 202.39% compared with the control group.Electrical conductivity of treatment group was obviously higher than that of the control group and the correlation between electrical conductivity and drying time was alike with the correlation between ATP production and drying time,which proved that drying method increased cell membrane permeability and ATP production.