研制了一种用于食品中葡萄糖快速检测的一次性电化学酶传感器。通过循环伏安法将电子媒介体硫堇电聚合在丝网印刷碳(SPCE)电极上,然后用壳聚糖二氧化硅溶胶凝胶包埋葡萄糖氧化酶,涂布于已修饰硫堇的丝网印刷碳电极表面,制成了新型葡萄糖生物传感器。实验表明该传感器响应快、灵敏度高、稳定性好,在对葡萄糖测定中表现出良好的响应特性,并具有抗柠檬酸、抗坏血酸、苯甲酸钠、蔗糖、果糖等干扰的特点。在葡萄糖浓度为5.2×10-5～2.5×10-3mol/L,酶电极的响应电流的变化值与葡萄糖的浓度呈良好的线性关系,线性方程为Y=0.049 01+2.729 32x,最低检出限为4.96×10-6mol/L。

A novel electrochemical enzyme biosensor for determintion of glucose in food was developed by poly(Thionine) and glucose oxidase on the Screen-printed electrode by chitosan organic and inorganic sol-gel.The biosensor shows fast response,high sensitivity and good stability.In addition,the biosensor operated at low potential would be interference free from ascorbic acid,citric acid,sodium benzoate,sucrose,fructose.Under optimal experimental conditions,The linear response of the sensor to glucose was in the concentration range of 5.2×10-5-2.5×10-3 mol/L with a detection limit of 4.96×10-6 mol/L.