以黄精为原料,采用单因素和正交试验研究浸提温度、料液比和时间对水浸出物、多糖和皂苷提取量的影响。单因素实验得出:影响较为显著的因素是浸提温度和料液比,时间对综合提取影响较小;正交试验分析得出:提取最佳工艺条件为浸提温度80℃,料液比1∶30(g∶mL),浸提时间3 h。在此优化条件下,水浸出物、多糖和皂苷提取量为67.92%,25.82%和6.64%。

Polygonatum were used as material,the effects of extraction temperature,ratio of solid to solution and extraction time on the extraction of Polygonatum water extract,polysaccharides and saponin were investigated by using single factor design and orthogonal design.The result showed that extraction temperature and ratio of solid to solution were more distinct factors than extraction time.the optimum extraction conditions by orthogonal design analysis is as follows: extraction temperature 80℃,the ratio of solid to solution 1g∶ 30mL,extraction time 3h.Under this condition,the extraction amount of Polygonatum water extract,polysaccharides and saponin were 67.92%,25.82% and 6.64%,respectively.