利用超滤对粘杆菌素发酵液进行膜分离处理并对其工艺过程进行优化。根据膜材料特性,选择聚醚砜(PES)为基材的超滤膜,通过不同截留分子量超滤膜的分离性能比较、操作条件选择,最终确定PES-10(截留分子质量为10 000 u)为超滤膜件,适宜的操作压力为0.3 MPa、料液pH值为6。经过超滤处理,滤液蛋白质含量为4.6mg/100mL,蛋白质去除率为96%,吸光度为0.185,发酵液中的色素得到有效地去除。

Ultrafiltration was used to pretreat colistin fermentation broth.According to the characteristics of the material and comparison of their ultrafiltration separation performance,polyether sulphone ultrafiltration membrane(MWCO=10 000) was selected.The optimum operating parameters had been determined: pressure was 0.3 MPa,pH was 6.0.Under these optimum parameters,the protein rejected ratio was 96%,absorbency was 0.185,and part of the pigment had been removed.