从黄伞子实体中提取出水溶性粗多糖,经DEAE Sepharose Fast Flow和SuperdexTM 200柱层析分离纯化得到一种多糖组分PAP2。采用高效凝胶渗透色谱法(HPGPC)检测其为均一多糖组分,平均分子质量为2.1×106u;经高效液相色谱-蒸发光散射(HPLC-ESLD)检测表明PAP2主要由葡萄糖组成;结合红外光谱、核磁共振1H NMR和13C NMR分析表明,黄伞子实体多糖PAP2是一种α-D-吡喃葡聚糖,主链为1-4糖苷键,存在1-6糖苷键的支链。

A water-soluble polysaccharide(PAP2) was obtained by purifying from the fruiting bodies crude polysaccharides of Pholiota adiposa through DEAE Sepharose Fast Flow ion-exchange column chromatography and SuperdexTM 200 column chromatography,respectively.HPGPC showed that PAP2 was homogeneous polysaccharide with an average molecular weight of 2.1×106 Da.Monosaccharide analysis by HPLC indicated that it was predominantly composed of glucose(Glc).Combination of IR,1H NMR and 13C NMR analysis showed that PAP2 owned characteristic infrared absorption peaks of polysaccharide and was a branched polysaccharide with a backbone of α-1,4-linked glucose and attached at position 6 of the branching residues.