食品与发酵工业

灵芝孢子粉多糖的PMP-HPLC指纹分析

  • 王浩豪 ,
  • 戴军 ,
  • 陈尚卫 ,
  • 朱松
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网络出版日期: 2011-12-25

Analysis of PMP-HPLC Fingerprints of Polysaccharides From Ganoderma lucidum Spores

  • Wang Hao-hao ,
  • Dai Jun ,
  • Chen Shang-wei ,
  • Zhu Song
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Online published: 2011-12-25

摘要

采用部分酸水解耦合PMP柱前衍生反相高效液相色谱法对15个不同灵芝(赤芝)孢子粉样品中多糖进行检测和指纹表征,研究建立灵芝孢子粉多糖的HPLC指纹图谱。标定了19个共有特征指纹峰,同一产地不同批次产品间的相似度均大于0.97,不同产地产品间的相似度大于0.8;赤芝孢子粉与其他不同部位及不同品种的灵芝类产品的多糖指纹图谱显示出较大的差异。这些相似性和差异可作为灵芝孢子粉产品质量监控、与其他不同部位和不同品种的灵芝类产品的区别鉴定以及灵芝孢子粉的真伪与掺假检测的主要依据之一。

本文引用格式

王浩豪 , 戴军 , 陈尚卫 , 朱松 . 灵芝孢子粉多糖的PMP-HPLC指纹分析[J]. 食品与发酵工业, 2011 , 37(12) : 133 -136 . DOI: 10.13995/j.cnki.11-1802/ts.2011.12.014

Abstract

Partial acidic hydrolysis coupled PMP-HPLC analysis was applied to indentify and characterize polysaccharides from Ganoderma lucidum spores from different zones in China.Chromatographic fingerprint of the polysaccharide was built using 15 different sources of Ganoderma lucidum spore powder samples and 19 common peaks were found.The similarity between products from the same zone was much higher than 0.97,while it was lower but still greater than 0.80 when the products originated in different zones.There are quite a lot of differences among Ganoderma lucidum spore and other species spore and between spore and fruit body of Ganoderma lucidum.The similarities and differences may be used as main basis for quality control,identification from different origins and parts of Ganoderma and their adulteration detection.
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