为进一步开发利用橄榄中的酚类物质,文中通过测定橄榄酚类提取物的铁离子还原能力、亚铁离子螯合能力,及对2,2'-氨基-二(3-乙基-苯并噻唑啉-6-磺酸)自由基(ABTS+.)、1,1-二苯-2-苦基苯肼自由基(DPPH.)、羟基自由基(.OH)和超氧阴离子自由基(O2-.)的清除能力,以考察橄榄酚类提取物的体外抗氧化性能,并与食品抗氧化剂丁基羟基茴香醚(BHA)和Vc进行对比。结果表明:橄榄酚类提取物具有较好的体外抗氧化性能,其ABTS+.、DPPH.清除率分别高达99%和95%以上,.OH、O2-.清除率可达50%左右,铁离子还原力约1 700μmol/L Trolox,亚铁离子螯合率最高为14.9%。且提取物ABTS+.、DPPH.和.OH清除能力及铁离子还原能力均强于BHA和Vc,而O2-.清除能力强于BHA但弱于Vc。另外,橄榄酚类粗提物和纯化物相同浓度下的ABTS+.、DPPH.、.OH清除能力和铁离子还原能力相当(P>0.05),而其O2-.清除能力和亚铁离子螯合能力表现有所差异。

Reducing power,metal chelating ability and free radicals scavenging ability were selected as the three indicators of antioxidant capacity of polyphenols from Canarium album(Lour.).BHA and Vc were used as positive control.The results showed that the polyphenol extracts had strong antioxidant capacity.The ABTS and DPPH radical scavenging rates were higher than 99% and 95%,while the hydroxyl and superoxide anion radical scavenging rates were as high as 50%.The FRAP showed a TEAC of 1700μmol/L Trolox.In addition,the Fe2+ chelating rate was up to 14.9%.The ABTS,DPPH and hydroxyl radical scavenging ability and iron reducing power of extracts were stronger than BHA and Vc.The superoxide anion radical scavenging ability of extracts was weaker than Vc,but stronger than BHA.The crude extracts and purified extreacts showed no difference on ABTS,DPPH and hydroxyl radical scavenging ability,They all similar with iron reducing power.However,the difference on superoxide anion radical scavenging and iron chelating abilities were obvious.