从新疆伊犁察布查尔县的土壤中分离到1株优势菌,该分离株生长特性与A型肉毒梭菌(Clostridiumbotulinum type A)一致,为革兰氏阳性粗大杆菌,在EYA培养基上菌落边缘呈锯齿状,表面粗糙不规则,有较大的乳浊环。根据分离株的形态、生理生化特性,结合琼脂糖凝胶凝胶电泳图像、16SrDNA V6-V8序列(GenBank登录号:JN248616)与系统发育树分析,将其鉴定为A型肉毒梭菌。

The dominant strain(GenBank accession number: JN248616) was isolated from the soil in Qapqal County,Xinjiang.Its growth characteristics were consistent with Clostridium botulinum of type A which belonged to thick gram-positive bacillus.Colonies in EYA culture medium showed serrated edge and irregular rough surface,with large opaque rings.Based on morphology of the strain,characteristics of physical and biochemistry,and combined with gel electrophoresis,16S rDNA sequences and phylogenetic tree,the strain was identified as Clostridium botulinum of type A.