对以糖蜜为原料培养高核酸酵母NY-1的发酵培养条件进行了优化。由实验得知,培养基最适初始pH为5.0,采用酵母浸粉和尿素的复合氮源,菌体生长较好;提高糖蜜中蔗糖水解率,可以延长酵母生长的对数期3h,有利于提高核酸酵母的浓度;采用挡板瓶提高溶氧后培养该酵母,菌体干重可达到15.4 g/L。

Sugar molasses was used to culture the High nuclear Saccharomyces.The fermentation condition was studied.The optimum medium ingredients and culture condition were obtained as followed:the initial pH 5.0,the complex nitrogen sources of yeast powder and urea;by increasing the rate of the hydrolyzation of the sucrose,the logarithm period of NY-1 is prolonged and the cell density was also increased.The cell dry weight was up to 15.4g/L when the yeast was cultured in baffled flasks.