在模拟大肠环境(厌氧和37℃)下,利用健康成人粪便提取物发酵粗玉米抗性淀粉,研究短链脂肪酸的产生情况,并剖析外源乳酸菌对产酸模式的影响。实验结果表明,粪便提取物单独发酵抗性淀粉时,短链脂肪酸含量随发酵时间增加而呈增加趋势;粪便提取物和2株乳酸杆菌共同发酵粗玉米抗性淀粉时,发酵产物中乙酸含量增加,丙酸和丁酸含量没有明显变化;当粪便提取物和2株乳酸球菌共同发酵粗玉米抗性淀粉时,发酵产物中丙酸和丁酸含量明显增加,乙酸含量没有明显变化。研究结果表明,外源性乳酸菌影响粗玉米抗性淀粉的模拟肠道发酵产酸模式。

At the stimulated conditions of the large intestines(anaerobic and 37℃),water extract of health adult feces was used to ferment a crude preparation of corn resistant starch.The amount of four short chain fatty acids in the fermentation system was measured to investigate the effects of four strains of exogenous lactic acid bacteria on the acid production.The results showed that the fecal extracts could ferment the resistant starch to produce four short chain fatty acids,which showed increased amount as the increase of the fermentation time.When fecal extract and one strain of Lactobacillus spp.were used to ferment the resistant starch,the amount of acetic acid in the fermentation system was enhanced while that of propionic or butyric acid was not impacted significantly.If fecal extract and one strain of Lactococcus spp.were used to ferment the resistant starch,the amount of propionic or butyric acid in the fermentation system was increased but that of acetic acid was not impacted.It was revealed that the exogenous lactic acid bacteria can influence on the acid production profiles of the resistant starch during its simulated fermentation in vitro.