建立了利用液液萃取技术分离酶法制备α-环糊精反应体系中复合剂——癸醇的方法。首先,在α-环糊精溶液中加入癸醇形成α-环糊精-癸醇复合物沉淀后,以该混合体系为研究对象,考察了萃取剂种类及其用量、萃取温度和萃取时间等因素对萃取法分离癸醇的影响,确定了萃取条件:以15倍于癸醇体积的乙醚作为萃取剂,在30℃下萃取30 min。其次,以酶法制备α-环糊精的反应体系为研究对象,在最佳萃取条件下分离癸醇后,所测得的α-、β-、γ-环糊精收率与采用传统方法——水蒸气蒸馏法所得收率相当,进一步验证了萃取法能够将α-环糊精-癸醇复合物中的癸醇有效分离出来。

An extraction method was developed to separate the complex reagent 1-decanol in enzymatic reaction system for α-cyclodextrin production.Firstly,a certain amount of 1-decanol was added to α-cyclodextrin solution to form α-cyclodextrin-1-decanol complexes for the parameter study of extraction separation.Effects of type and volume of extraction solvent,extraction time,and extraction temperature on extraction separation of 1-decanol were investigated.The results showed that the optimum conditions were that ethyl ether,15 times as many as the volume of 1-decanol,was used as extraction solvent to extract 1-decanol at 30℃for 30 min.Secondly,under above optimum conditions,1-decanol in enzymatic reaction system for α-cyclodextrin production was extracted.The results showed that,after extraction separation of 1-decanol,α,β and γ-cyclodextrin yields determined were almost the same as those by the traditional separation method——steam distillation,which further verified that 1-decanol in the α-cyclodextrin-1-decanol complexes could be effectively separated using extraction method.Furthermore,compared with steam distillation,the proposed extraction method was simple,energy-saving and efficient.