通过单因素和正交实验,优化了以灵芝菌发酵紫甘薯渣生产可溶性膳食纤维的发酵培养基和培养条件,并且进行了发酵罐放大实验。在摇瓶水平,采用紫甘薯渣4 g,豆渣1 g,料液75 mL,pH 6.0,接种量16%,甘蔗渣2%,KH2PO40.1%、MgSO4·7H2O 0.05%、VB10.005%,发酵4天,可溶性膳食纤维达到15.89 g/L。相同条件下,15 L发酵罐中,通气量200 L/h,转速为50 r/min,装液量65%,可溶性膳食纤维达到14.73 g/L。采用优化工艺,发酵前紫甘薯渣中可溶性膳食纤维含量提高了10.92 g/L。

Investigate the optimal fermentation conditions of SDF fermented by Ganoderma lucidum used purple sweet potato slag which provided the scientific proof for the effective utilization of purple sweet potato slag.Fermentation medium and condition of culture were optimized by single-factor test and orthogonal test.In addition,magnified experiment was conducted.By using the shake-flask test method and 4g purple sweet potato slag,1g bean dregs,75 mL liquid materials,pH 6.0,inoculums amount 16%,bagasse 2%,KH2PO4 0.1%,MgSO4·7H2O 0.05%,VB1 0.005%,fermentation time 4d,the SDF could reach 15.89 g/L in the above described culture medium.Under the same conditions,and adopting ventilation 200 L/h,speed 50r/min,fluid volume 65%,the SDF content could reach 14.73g/L.Through the optimization of process conditions,the SDF was improved by 10.92g/L compared with the content of slag purple sweet potato that had not been fermented.