采用回归正交实验优化了杏鲍菇中多糖的微波辅助提取工艺条件;在单因素实验基础上根据Box-Be-hnken中心组合实验设计原理,进行4因素3水平二次回归正交实验,依据二次回归线性分析确定各工艺条件的影响因素。结果表明:杏鲍菇子实体多糖提取的适宜工艺条件为提取温度76℃,提取时间12 min,料液比1∶35,微波功率700W,提取2次,在此条件下杏鲍菇多糖的得率可达11.80%。

The extractions of microwave enhancement extraction of polysaccharide from fresh Pleurotus eryngii were optimized by orthogonal regression design.On the basis of single-factor experiments and the principles of Box-Behnken central composite experimental design,the quadric regression orthognal optimize experiment with 4 factors and 3 Levels were used,the quadric regression linear analysis methodology were used to explore the effect of each factor on extraction rate of polysaccharide.The results shows that the optimal extraction processing conditions were: temperature 76℃,microwave time 12 min,material-water ratio 1∶35(g∶mL),microwave power 700 W and extract twice.Under the optimal extraction conditions,the extraction rate of polysaccharide from Pleurotus eryngii's fruit-body was up to 11.80%.