内部转录间隔区(internal transcribed spacer,ITS)序列是目前最常用的酵母分子鉴定标识之一。该文利用ITS序列同源性分析法对保藏于中国高校工业微生物资源与信息中心的623株酵母分离物进行了鉴定。实验结果显示:581株酵母分离物(93.3%)可通过ITS序列直接鉴定到种一级,40株分离物(6.4%)可鉴定至属一级,仅有2株酵母分离物无法通过ITS序列获得有效鉴定。上述结果表明,ITS序列在大多数酵母种属鉴定中具有很高的敏感性和特异性,可以作为第一分子标识用于大批量酵母分离物的分子鉴定。

The internal transcribed spacer(ITS) region of nuclear DNA is one of the most used locus for yeast identification with growing sequence data deposited in public-access databases.This study extensively evaluated the feasibility of ITS sequence analysis for systemic classification of yeast isolates.The ITS regions of 623 yeast strains deposited in CICIM-CU were amplified by PCR and sequenced,the sequences were compared to reference data available at the GenBank database by using BLAST.Most of fungal isolates(93.3%) were assigned to species level successfully.40 strains(6.4%) were only can be assigned to genus level.2 strains cannot be identified based on ITS sequences analysis.This study shown ITS sequences were sufficiently sensitive for identifying most of yeasts,it can be powerfully and ordinarily used as first-round barcoding gene to systemic identification of bulk yeast isolates.