对在30 L发酵罐中雪白白僵菌发酵环孢菌素A的分批补料发酵过程进行了动力学研究。通过对环孢菌素A分批发酵数据进行分析,基于Logistic模型和Luedeking-Piret方程,建立了环孢菌素A发酵动力学模型,包括细胞生长、底物消耗和产物合成回归方程。应用Origin7.5软件对模型进行非线性拟合计算,建立的动力学模型与实验值拟合良好,能较准确反映环孢菌素A分批补料发酵动力学过程。

The kinetic models of the fed-batch fermentation of cyclosporin A in 30l bioreactor were studied.Based on the fed-batch fermentation data and Logistic model,the kinetics models of cyclosporin A fermentation for cell growth,cyclosporin production and substrate consumpsion were built up.The program of origin 7.5 version was used to fit the model.The analysis results showed that the good agreement of predicted values with the experimental values,and that the kinetic models could provide reasonable descriptions for the process of cyclosporin A fed-batch fermentation.The development of fed-batch fermentation kinetics of cyclosporine A would help to regular the fermentation of cyclosporine A and increase the fermentation titer.