研究了木薯酒精发酵中,蒸馏废液回用比分别为30%(v/v),50%(v/v),70%(v/v)和90%(v/v)时对酒精发酵的影响,循环回用实验共进行11批。结果表明,循环回用比例为30%(v/v)时对酒精发酵略有影响;当循环回用比例≥50%(v/v)时,随着循环批次的增加,酒精产量下降。蒸馏废液中,酿酒酵母及污染杂菌产生的代谢副产物(如有机酸、甘油等)的累积是造成酒精产量下降的主要原因。而蒸馏废液中氨氮的累积造成了发酵液颜色的加深。

The effects on ethanol fermentation with cassava is studied,when the recycling proportion of distillery waste is 30%,50%,70% and 90% respectively.Up to eleven fermentation cycles were conducted.The results showed that,the slight influence were found in alcohol fermentation when recycling proportion is 30%,when recycling proportion is above 50%(include 50%) ethanol yield descended with the increasing of recycles.The accumulation of metabolic by-products such as organic acids,glycerin produced by Saccharomyces cerevisiae and other contamination bacteria in the distillery waste is the main reason,which Reduce the ethanol yield,The accumulation of ammonia nitrogen in the distillery waste fermentation also caused the color to deepen.