以苦菜中提取的叶绿素为原料,经皂化、酸化、铁代、成盐制备叶绿素铁钠盐,用紫外-分光光度法检测其质量。单因素试验和响应曲面法结合优化工艺试验条件为:先在pH≈12的体系中,59℃下皂化90 min;再于pH≈2～3体系中,65℃下酸化60 min后,加入42 mL质量分数10%FeSO4溶液,铁代时间97 min;最后用10%NaOH-乙醇调节溶液pH≈12而成盐。

The sodium iron chlorophyllin by the chlorophyl form Sonchus oleraceus L was obtained through the following successive steps: saponificatioan,acidification,replacement of sodium by iron,and neutalization.Its quality was evaluated by UV-spectrophotometer.Single factor experiment and response surface method in optimization of process test conditions: Firstly,chlorophyl is saponifieated for 90min at 59℃ in the system with pH≈12;Secondly,the saponificated production is acidificated for 60min at 65℃ in the system with pH≈2~3;And afterword replaces sodium with 42mL 10%FeSO_4 for 97min.Finally,the sodium iron chlorophyllin is obtained by neutralizing at pH≈12 with 10%NaOH-ethanol.