采用反相高效液相色谱法分析测定苹果酸发酵液中的有机酸,最终确定了适用于发酵液中各种有机酸含量测定的色谱条件,色谱柱:COSMOSIL 5C18-PAQ(4.6 mm I.D.×250 mm);紫外检测波长:210 nm;流动相:1%乙腈-20 mmol/L KH2PO4溶液(pH 2.8);流速:0.5 mL/min;柱温:23℃。结果表明,在该条件下苹果酸发酵液中的7种有机酸都得到了较好分离,方法的回收率为94.2%～102.7%,表明具有较好的准确度和精密度。

Reversed phase HPLC method was applied to determine organic acid in malic acid fermentation liquor in this paper.The chromatographic conditions are finally conformed to apply to all kinds of organic acid content determination in the fermentation.The conditions are as follows: COSMOSIL 5C18-PAQ column(4.6 mm I.D.× 250 mm) was used at 23℃.UV detection wavelength was 210 nm.Solution containing 1% acetonitrile and 20mmol/L KH2PO4 at pH 2.8 was used as mobile phase with flow rate of 0.5 mL/min.The seven organic acids in malic acid fermentation liquor were preferably separated under these conditions.The recovery ratio was 94.2%～102.7% and the method had good accuracy and precision.