利用热激的β-乳球蛋白(β-Lg)与矢车菊素-3-葡萄糖苷(C3G)共组装形成纳米粒以保护C3G的抗氧化活性。溶液pH值为2.5～6.5、蛋白热激温度为30～85℃及β-Lg与C3G不同摩尔比例(1∶2～1∶32)对纳米体系的粒径、Zeta电位、包埋率及单位蛋白载药量具有显著影响。在pH 6.5时能够形成稳定的纳米分散体,在热激温度85℃、β-Lg与C3G摩尔比为1∶2时,β-Lg对C3G的抗氧化活性具有最好的保护作用。

Cyanidin-3-glucoside(C3G) was coated by heat treatment with β-lactoglobulin(β-Lg) for the preservation of antioxidant activity.The effects of pH(2.5-6.5),heating temperature of β-Lg(30-85 ℃) and the molar ratio of β-Lg to C3G(1∶ 2-1∶ 32) on the properties of β-Lg-C3G complexes were studied.All three factors significantly influenced the particle size,zeta-potential,entrapment efficiency of C3G and C3G encapsulation with β-Lg particles.Stable and clear solution could be obtained at pH 6.5.The highest protection of EGCG antioxidant activity was obtained with β-Lg heated at 85 ℃ and the molar ratio of 1∶ 2(β-Lg∶ C3G).