以废弃啤酒酵母为原料采用催化自溶与生物破壁技术生产细胞壁多糖。经过单因素试验后确定了酵母自溶的适宜条件:50～60℃,pH 5.0～6.0,食盐浓度0.8%,VB2浓度200 mg/L,料液比(g∶mL)1∶8～10,剪切微化处理约9 h以上。此外,确定了采用60℃下,1∶10的料液比,0.02%～0.03%的蛋白酶添加量和2%的NaOH浓度为细胞壁多糖的适宜提取条件。此方法得到的细胞壁多糖中甘露聚糖的含量>22%,葡聚糖的含量>35%。同时发现使用酪蛋白钠和改性淀粉作为酵母提取物的微胶囊化材料时,产品喷雾效果好,且不吸潮。

This paper discussed the use of waste beer yeast to produce cell wall polysaccharides with catalytic autolysis and cell wall deconstruction technology.Through single factor experiments,the best autolysis efficiency of the yeast were as follows: 50～60℃,pH 5.0～6.0,0.8% NaCl and 200 mg/L VB 2,solid-liquid ratio 1∶(8～10),shearing treatment at least 9h.Also,the best extraction conditions for extraction polysaccharide were: 60℃,solidliquid ratio 1 /10,0.02%～0.03% protease and 2% NaOH.In this method,we can get at least 22% poly-mannose and 35% poly-glucan from the cell wall polysaccharides.Sodium casein and modified starch combination was the best microencapsulated material due to its good spray effect and moisture resistance.