考察了过氧化氢的添加及其浓度、提取温度、提取时间等因素对罗非鱼皮明胶的提取率和明胶黏度的影响。采用响应面实验设计优化显著影响明胶提取率和明胶黏度的工艺参数,确定最佳提取工艺条件。实验优化结果为过氧化氢浓度0.07 mmol/g、提取温度53℃、提取时间4.6 h,此条件下明胶提取率达87.71%,黏度达8.90(mpa·s)。

The effect of H2O2 concentration,extraction temperature,extraction time on the yield and viscosity of tilapia skin gelatin extraction were studied by one-factor-at-a-time experiment and Response Surface Methodology(RSM).Results showed that the optimum conditions were: H2O2 concentration 0.07mmol/g,extraction temperature 53℃ and extraction time 4.6 hour.Under these conditions,the gelatin yield and gelatin viscosity were 87.71% and 8.90(mPa.s),respectively.