在单因素试验的基础上,采用响应面法的BBD组合设计,对淫羊藿提取液清除亚硝酸盐的参数进行优化。淫羊藿中清除亚硝酸盐有效成分的提取工艺条件为:提取时间176 min,乙醇体积分数58%,提取温度78℃,液料比37∶1(mL∶g),此时提取液对亚硝酸盐的最大清除率为75.64%。
On the basis of single factor experiment,the parameters of scavenging effect on sodium nitrite by herba epimedii was optimized by response surface methodology(RSM) based on a box-behnken design(BBD).The study showed that the optimum extraction was: ethanol concentration 58%,ratio of material to liquid 1∶ 37;extraction time 176min and extraction temperate 78℃.The maximum capabilities of scavenging was 75.64%.
