首次采用同时蒸馏萃取法(SDE)提取了不同培养方式下获得的台湾虫草液体和固体菌丝体及人工培养子实体的挥发性成分,并应用气相色谱质谱联用仪(GC-MS)进行了分析。结果表明:不同培养方式对台湾虫草挥发性物质的数目和含量有较大影响,对挥发性成分的类型影响不大,主要为醇类、烯类、酯类、酚类和醚类化合物。液体和固体培养菌丝中分别鉴定出19种、12种挥发性物质,分别以1-辛烯-3-醇(51.63%)和2-癸烯-5-内酯(41.13%)的相对含量最高,人工培养子实体中鉴定出15种挥发性物质,以1-辛烯-3-醇的相对含量最高(37.70%)。3种人工培养物挥发性物质的共有成分为1-辛烯-3-醇、2-癸烯-5-内酯、2,6-二叔丁基对甲酚及4-羟基-3-叔丁基-苯甲醚。

The volatile components in mycelia of Cordyceps formosana cultured under different conditions and artificial culture of fruiting bodies of C.formosana were extracted by simultaneous distillation and extraction(SDE) for the first time and analyzed with gas chromatography-mass spectrometry(GC-MS) in this paper.The results showed that culture conditions had great influence on the content and number of volatile components,but little impact on compound classes which mainly were alcohols,olefins,esters,phenols and ethers.There were 19 and 12 volatile components identified in mycelia of liquid and solid culture with the highest content of 1-octene-3-alcohl(51.63%) and 2-decene-5-lactone(41.13%),respectively.Fifteen compounds are identified from artificial culture of fruiting bodies,wherein the content of 1-octene-3-alcohl was highest(37.70%).The common volatile components of 3 kinds of artificial culture of C.formosana were 1-octene-3-alcohol,2-decene-5-lactone,butylated hydroxytoluene and 3-tert-Butyl-4-hydroxyanisole.This paper will provide experimental foundation and scientific basis for further development of C.formosana.