蜂王浆经体积分数95%乙醇提取、MCI-Gel、ODS等反相色谱柱分离,得到单磷酸腺苷(AMP)和7种脂肪酸。采用1,1-二苯基苦基苯肼自由基(DPPH.)清除率和总抗氧化能力试剂盒(T-AOC)等方法探索蜂王浆醇溶性物质的抗氧化能力,结果表明:蜂王浆醇提后抗氧化活性提高,分离得到的AMP和3,10-二羟基癸酸(3,10-DDA)的抗氧化性相对较强,且清除DPPH.的IC50值分别为6.274 mg/mL和9.153 mg/mL,是蜂王浆中的抗氧化活性物质。

After royal jelly(RJ) was extracted with 95% ethanol and isolated with chromatographic columns MCI-Gel and ODS,adenosine monophosphate and 7 kinds of fatty acids were obtained.The scavenging free radicals(DPPH·)of 1,1-diphenyl-2-picrylhydrazyl and the total antioxidant capacity kit(T-AOC)were used to study antioxidation of alcohol soluble components.The results showed that antioxidation of royal jelly improved after extraction and the antioxidant activity of AMP and 3,10-dihydroxydecanoic acid(3,10-DDA)were relatively higher,IC50 value for DPPH· scavenging activity were 6.274 mg/mL and 9.153 mg/mL respectively.They were identified as the Antioxidants in royal jelly.