A strain SK19.001 which could produce poly γ-glutamate(γ-PGA) independent of glutamic acid in culture was isolated.It was identified as Bacillus methylotrophicus based on the sequence analysis of 16S rDNA and a series of morphological and biochemical characteristics.The yield of γ-PGA was 14.57 g/L when the strain was grown in glycerol and peptone F403-containing medium at 37℃ for 24 h with shaking.
