效应面法优化枇杷叶总黄酮的提取工艺。以乙醇浓度、料液比和提取时间为自变量,总黄酮提取率为因变量,通过自变量与因变量的完全二次响应面的回归拟合,用效应面法选取最佳工艺,并进行预测分析。结果表明:适宜的提取工艺是,提取液乙醇的体积分数为73%,料液比1∶11.5(g∶mL),提取2次,每次140 min。

To optimize the process of extracting effective constituents from Folium eriobotryae by response surface methodology.The independent variables were ethanol concentration,extracting time and solvents ratio.The dependent variable was extracting rate of total flavonoids.Composite design and response surface methods were used to optimize the extracting process,a two-order polynomial equation was fitted,and model was used to predict the response in the optimal region.the optimum conditions of extraction process were:73%% ethanol,140 minutes for reflux,11.5 fold solvent and 2 times for extraction.The optimum model is highly predictive.