利用水提醇沉法提取黄精多糖肽,三氯乙酸法、透析法和阴离子交换柱层析法分离纯化黄精多糖肽;GC-MS分析了黄精多糖肽的单糖组成;Fenton法、邻苯三酚自氧化法和磷钼络合物法分别研究了黄精多糖肽对羟基自由基、超氧阴离子清除和总抗氧化能力。结果表明:黄精中多糖肽的含量为0.34%,多糖肽中多糖和蛋白质的比率为5.4∶1;黄精多糖肽的单糖组成主要是鼠李糖、阿拉伯糖、D-木糖、甘露糖、葡萄糖和半乳糖等,其相对百分含量分别为:2.12%、1.81%、2.51%、65.4%、14.3%、13.8%。黄精多糖肽在一定范围内随着浓度的增高对羟基自由基的清除率、对超氧阴离子的清除率逐渐增高,总抗氧化活性也随黄精多糖肽浓度的增高而增高;其对羟基自由基和超氧阴离子的清除率高于Vc,但总抗氧化活性远低于VC的总抗氧化活性。

Polygonatum glycoprotein was extracted from polygonatum by hot water and sinking in alcohol.The trichloroacetic acid,the dialysis and the anion-exchange column chromatography were used to purify the Polygonatum glycoprotein.Monosaccharide composition of polygonatum glycoprotein was studied by GC-MS and the antioxidant activity of Polygonatum glycoprotein were studied by Fenton oxidation,phosphomolybdenum complex method and pyrogallol autoxidation method.The results showed that: The content of Polygonatum glycoprotein was 0.34%,the ratio of polysaccharide and protein was 5.4∶ 1,the monosaccharide composition of Polygonatum glycoprotein mainly were rhamnose,arabinose,D-xylose,galactose,glucose and mannose polysugar and their relative percentage contents were: 2.12%,1.81%,2.51%,65.4%,14.3%,13.8%.Scavenging ratio of hydroxyl radical,scavenging ratio of superoxide anion radical and total antioxidant activity of polygonatum glycoprotein increased gradually by the contents of polygonatum glycoprotein.Scavenging ability of hydroxyl radical and superoxide anion radical were higher than that of Vc,however the total antioxidant activity was lower than that of Vc.