考察巴氏醋杆菌Ap2012在不同碳源酵母膏平板的生长情况及其对乙醇、糖、酸、NaCl的耐受性。结果表明,在酵母膏平板上,Ap2012能利用葡萄糖、乙醇作为碳源生长并产酸,能较好地利用甘油、果糖、蔗糖、山梨糖,对甘露醇的利用较差。乙醇体积分数为12.4%时巴氏醋杆菌Ap2012的生长受明显抑制,达到15.8%时转酸能力受明显抑制。葡萄糖的质量浓度达到300 g/L,NaCl的质量浓度为15 g/L时,巴氏醋杆菌Ap2012的生长和转酸能力受到明显抑制。乙酸质量浓度达到42 g/L时,巴氏醋杆菌Ap2012的生长受到明显抑制。结果表明,巴氏醋杆菌Ap2012对高浓度糖及乙醇有较好的耐受性,对乙酸和NaCl的耐受性较差。

Growth and tolerance of Acetobacter pasteurianus were mainly studied in this paper.Growth of Acetobacter pasteurianus Ap2012 on different carbon sources and its tolerance to ethanol,glucose,acetic acid and NaCl were determined.Ap2012 could utilize glucose and alcohol as carbon sources and produced acetic acid.Glycerol,fructose,sucrose,and sorbitol could be used as good carbon sources.Ap2012 couldn't grow well when mannitol was used as a carbon source.Growth of Ap2012 could be inhibited by 12.4% ethanol.Acetic acid conversion ability of Ap2012 was inhibited by 15.8% ethanol.Both the growth and acetic acid conversion ability of Ap2012 were inhibited by 300g/L glucose or 15g/L NaCl.Growth of Ap2012 was inhibited by 42 g/L acetic acid.It showed that Ap2012 had good tolerance to high content of sugar and alcohol,and bad tolerance to acetic acid and NaCl.