食品与发酵工业 >

2012 , Vol. 38 >Issue 10: 120 - 122

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2012.10.015

食品与发酵工业

枯草芽孢杆菌凝乳酶高产菌株的微波诱变

  • 梅林 ,
  • 陈芳 ,
  • 阚睿 ,
  • 徐鹏
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网络出版日期: 2012-10-25

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The Breeding of the Rennet High-producing Strain from Bacillus subtilis and Studies on Conditions for Microwave Irradiation

  • Mei Lin ,
  • Chen Fang ,
  • Kan Rui ,
  • Xu Peng
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Online published: 2012-10-25

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摘要

以1株产凝乳酶枯草芽孢杆菌菌株为对象,对其进行微波诱变处理,考察诱变时间、诱变频率对菌株诱变效果影响,得到最佳诱变条件为:诱变时间为4 min,诱变功率为450 W;获得1株高凝乳酶活菌株(菌株编号为BsB1.1),其遗传稳定性良好,凝乳酶活达到516.12 U/g,比出发菌株提高了29.0%。

关键词： 枯草芽孢杆菌; 凝乳酶; 微波诱变; 诱变时间; 诱变功率

本文引用格式

梅林 , 陈芳 , 阚睿 , 徐鹏 . 枯草芽孢杆菌凝乳酶高产菌株的微波诱变[J]. 食品与发酵工业, 2012 , 38(10) : 120 -122 . DOI: 10.13995/j.cnki.11-1802/ts.2012.10.015

Abstract

After the strain of Bacillus subtilis was treated with Microwave Irradiation,a high rennet activity strain was obtained.Rennet activity produced by BsB1.1 reached 516.12U/g,which was increased by 29% compared with original strain.The tests of microwave irradiation conditions showed that the optimal time was 4min and the optimal power was 450 W.

Key words： Bacillus subtilis; chymosin; microwave irradiation; microwave irradiation conditions

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