为揭示环境因素对屎肠球菌产苯乙胺和酪胺的影响,研究了NaCl、糖的添加量、pH、含氧量、温度环境因素以及产胺菌之间对生物胺的影响。结果表明,当pH为5.00时,屎肠球菌的生长和产生物胺能力都受到抑制。当pH为6.00时,产胺菌的产胺能力在有氧条件下显著低于厌氧条件下。通过4因素二次回归方程分析pH、温度、加糖量和加食盐量对生物胺产生的影响,结果表明,pH、糖、盐和温度对屎肠球菌生长有显著的影响,其中受pH和温度影响较大。pH和温度对苯乙胺和酪胺产生有显著的交互影响,并且这种交互作用随着温度升高显著增强。

Environmental factors such as pH,oxygen content,temperature,addition of NaCl and sugar and effects of amines-productive dominant bacteria on biogenic amines were studied in order to find out production mechanism of phenylethylamine and tyramine by Enterococcus faecium.The results showed that the growth rate and production ability of phenylethylamine and tyramine-production of Enterococcus faecium were inhibited at initial pH 5.00,and production ability of phenylethylamine and tyramine-production was lower at aerobic environment than that at anaerobic ones.Temperature,pH,addition level of NaCl and sugar had significant effect on growth rate of Enterococcus faecium and the effect of pH and temperature was higher by four factors quadratic rotation experiment.There were significant interactional effect of pH and temperature on production of phenylethylamine and tyramine.There were significant interactional effects of addition level of pH and temperature on production of phenylethylamine and tyramine and the effects were also increased with the increase of temperature.