利用不同方法对荷叶黄酮进行提取,选择提取率较高的方法,采用DPPH自由基清除实验,比较抗氧化活性,利用中效原理探讨荷叶黄酮、VC单独和复配后的抗氧化作用。结果显示:丙酮提取法的黄酮提取率和纯度最高,荷叶黄酮和VC的IC50分别为92.89μg/mL和1 429.33μg/mL。当黄酮浓度小于138.05μg/mL,VC的浓度小于9.2μg/mL时2种样品呈协同作用,即表现出比单独使用更好地效果。

The flavonoids from Lotus leaves were extracted by different methods in order to select the best one.DPPH radical scavenging assay was used to explore their antioxidant ability.Based on median effect principle,the antioxodation effects for Lotus leaf flavonoids solely and combined with Vitamin C were tested.The results showed that the acetone extraction was the best with the highest extraction rate and purity.The IC50 of Lotus leaf flavonoids and VC were 92.89 μg/mL and 1 429.33 μg/mL.The synergistic effect showed when flavonoid concentrations was less than 138.05 g/mL and VC concentrations less than 9.2 μg/mL.