气生分生孢子是生防真菌应用和剂型化的主要有效成分之一,该研究对自行研制的具有应用潜力的串联式产孢箱发酵生产气生分生孢子的工艺参数进行了优化。通过对球孢白僵菌ChB9菌株在两种常用培养基质(籼米和粳米)以不同重量面积比、不同灭菌方式下发酵得到的孢子粉产量、气生菌丝产量和培养基利用率等指标的统计分析来优化该产孢箱在发酵生产气生分生孢子过程中的相关关键工艺参数。结果表明,粳米、105℃灭菌30 min与重量面积比(g:cm2)为1∶2的组合条件最符合产业应用实际,产量为17.56±2.79 g孢子粉/kg稻米,为最佳选择。该研究获得的优化参数不仅可以指导生防真菌孢子粉的小规模实验室生产,而且可为以麦类等其他发酵基质的发酵生产工艺优化提供参考。

Aerial conidia were one of key ingredient for formulation and application of fungal biocontrol agents.Statistical optimization strategy of technology parameters was adopted to maximize the conidia production through series fermentation chamber which have been previously developed to produce high-quality conidia powder.Beauveria bassiana ChB9 isolate was evaluated as fermentation candidate.Three important fermentation factors including culture substrates,sterilization conditions and rates of substrate weight and windrowing area(g∶ cm2) at varied combinations were used to study the optimal parameters required for series fermentation.Conidia powder yield,mycelium powder yield and rice consumption rate under different factors combination were compared.The combination japonica rice substrate with 105℃ 30 minutes sterilization under 1∶ 2 weight/area rate conferred best conidia powder yield of 17.56±2.79g per 1 kg rice substrate.Those results might be not only advantageous for further studies on laboratory-scale conidia production of biocontrol agents based on series chamber system,but also guidable for optimization of technology parameters on other substrate fermentation.