为研究食品中腐败酵母实时荧光PCR快速鉴定方法,设计和筛选出了可用于腐败酵母鉴定的多条探针和引物,并建立了针对酿酒酵母、鲁氏接合酵母、斯巴达克毕赤酵母和布鲁塞尔德克酵母等4种腐败酵母菌的实时荧光PCR鉴定方法。用文中建立的方法对从糕点、蜂蜜、饮料等市售食品中分离出的60余株酵母菌进行了鉴定,发现其中4株为酿酒酵母,21株为鲁氏酵母,其他为与上述4种不同的酵母。以实时荧光PCR方法鉴定酵母,全过程仅需约3 h,与常用的生化鉴定方法相比,简化了鉴定步骤,提高了鉴定准确性,缩短了鉴定时间。

To rapidly identify spoilage yeast isolated from food by real-time PCR,several specific probes and primers were designed or selected.The method for identification of Saccharomyces cerevisiae,Zygosaccharomyces rouxii,Dekkera bruxellensis and Spartak pichia by real-time PCR were established in this study.More than 60 strains of yeast isolated from commercial food were identified by real-time PCR method.The results showed that 4 strains of them were Saccharomyces cerevisiae,and 21 strains were Zygosaccharomyces rouxii,the other 35 strains didn't belong to that 4 species of yeast.To identify yeast by real-time PCR method,the whole process took only about 3 hours.Compared with the traditional biochemical method,real-time PCR method was more time-saving,sensitive,and accurate.