菊糖内切酶能催化菊糖不完全水解形成果寡糖。果寡糖具有许多独特的生理功能,例如调节肠道菌群、非致龋齿性、降低血清胆固醇、改善脂质代谢、提高机体免疫力且对人体无任何生理毒性等,是公认的益生元代表,因而受到广泛关注。文中简要论述了果寡糖的生理功能和生产方法,重点论述了菊糖内切酶活性测定方法、酶学性质、分子生物学,以及应用等方面的最新研究进展。

Endo-inulinase can selectively hydrolyze inulin to form fructooligosaccharides.Fructooligosaccharides has attracted much more attention in the industrial application due to its attractive features such as modifying gut microbial population,non-cariogenic,lowing total cholesterol and lipid in the serum,stimulating immune function and harmless to health,so it is known as prebiotics.In this paper,we discuss the functions and production of fructooligosaccharides,and particularly,we focus on the activity assay,properties,molecular biology,and application of endoinulinase,which is in favour of developing fructooligosaccharides in the application of foodstuff.