建立了一种快速、敏感、特异检测弓形菌的PCR方法。选取弓形菌gyrA基因序列作为靶序列设计特异性引物Arco/gyrAF和Arco/gyrAR,进行PCR扩增实验。结果弓形菌呈现出一条298 bp的特异性条带,其余非弓形菌均无阳性扩增。灵敏度实验结果表明,该法可以检测到82.6 pg的DNA。经过对16份生鲜鸡肉样品的检测,9份样品中检测到弓形菌,样品阳性率为56.25%。该PCR方法可以高效、灵敏地检测食品中弓形菌,具有很强的实际应用价值。

To establish a rapid,sensitive and specific method of polymerase chain reaction( PCR) for detection of Arcobacter species,a pairs of primers Arco / gyrAF and Arco / gyrAF were designed based on gyrA gene. After the amplification by PCR assay,Arcobacter had a positive reaction with 298 bp amplification fragments,while all the other strains just had a negative reaction. The result of sensitivity tests showed that the detection limit for DNA could reach82. 6 pg. Nine of 16 chicken( 56. 25%) samples were positive for Arcobacter using the PCR method. This PCR technique showed definitive advantages in terms of sensitivity and technical simplicity.