研究发酵方式对隐甲藻生长和积累DHA的影响,从而为工业化生产提供参考。对分批发酵初始葡萄糖浓度进行优化,得到最佳葡萄糖浓度为60 g/L。在此条件下培养9 d后DHA的产量为0.8 g/L。在分批发酵的研究基础上,在稳定期补加一次葡萄糖,每升培养基补加30 g,培养9 d后DHA产量达到1.27 g/L。如果每隔24 h补加1次葡萄糖使培养基中葡萄糖的浓度恢复到20 g/L,培养9d后DHA产量达到1.72 g/L。结果表明,在隐甲藻产DHA实验中补料分批发酵要优于分批发酵。

In order to provide reference for industrialized production of DHA,the effect of fermentation mode on the growth of C. cohnii and accumulation of DHA was studied. After optimization of the initial concentration of glucose during batch fermentation,we got the maximal DHA production when the concentration of glucose was 60g / L. Under this condition,the DHA yield reached 0. 8g / L after cultivating for 9 days. Based on the research of batch fermentation,30g glucose per liter of medium was fed once during stationary phase,the DHA yield reached 1. 27g / L after cultivating for 9 days. What's more,when glucose was fed once every 24 hours to keep the concentration of glucose at20g / L,the DHA yield reached 1. 72g / L after cultivating for 9 days. Results showed that fed-batch fermentation was superior to batch fermentation in the accumulation of DHA in C. cohnii.