在单因素试验的基础上,采用正交L9(34)试验法,以红腺忍冬花为原料,优化其绿原酸的微波提取工艺.考察微波功率、料液比、提取次数、提取时间和溶剂pH值对提取效果的影响,实验结果表明:红腺忍冬绿原酸的最佳微波提取工艺条件为按1∶12(g∶mL)的料液比加入pH 3的水溶液进行微波辅助提取2次,每次5 min,红腺忍冬山银花中绿原酸提取率可达到4.54%,与传统提取方法相比,该工艺具有提取时间极短、耗能少和提取较高等优点。

The extracting process of chlorogenic acid from Lonicera hypoglauca Miq. with microwave-assisted method was optimized. Oahogonal design L9( 34) method was applied to study the effect of five factors such as microwave power,ratio of material / solvent,extraction duration,time of extraction and solvent pH on the extraction efficiency based on the experimental result of single factor method. The results have shown that optimum conditions of microwave power,solvent pH,ratio of material / solvent,extraction duration and time of extraction were 400W,pH 3,1: 12,5 min and 2 times,respectively. The extraction rate of chlorogenic acid from Lonicera hypoglauca Miq was up to 4. 54% under the optimum extracting conditions. The method developed in the paper has the advantages of simple operation,higher extraction efficiency and very short extraction duration which is appropriate for the extraction of chlorogenic acid from Lonicera hypoglauca Miq.