采用新型常压室温等离子体射流诱变拟无枝酸菌CCTCC M2011265(Amycolatopsis sp.),在等离子体对菌株致死率为61.71%的条件下,以香兰素产量为指标的正突变率达到17.6%,其中复筛得到遗传稳定性的高产突变株LQ-6,香兰素产量达到6.78 g/L,对前体阿魏酸的摩尔产率为72.14%,比出发菌株提高了21.29%,同时其香兰素合成代谢途径中的关键酶脱乙酰酶活性提高了43.2%,香兰素氧化酶活性降低了38.7%。

To obtain vanillin mutants with improved vanillin production,an atmospheric and room temperature plasma( ARTP) jet was used to screen high vanillin-producing Amycolatopsis sp. strains by a 96-well culture plate. The positive mutation rate of the strains was 17. 6% under the condition of lethality rate of 61. 71%. A high yield mutant LQ-6 with good genetic stability was obtained. It could produce 6. 78g / L vanillin while a molar yield of ferulic acid arrived 72. 1%,which was 21. 3% higher than that of the original strain. Moreover,the deacetylase activity was enhanced by 43. 2% in LQ-6,while the vanillin oxidase activity was decreased by 38. 7% compared with the original strain.