从甘蔗汁中筛选出1株利用葡萄糖产甘露醇的菌株,经鉴定为近平滑假丝酵母(Candida parapsilosis)。研究其发酵生产甘露醇的条件,通过单因素实验和正交实验优化后,培养基配方为:200 g/L葡萄糖,30 g/L酵母膏,0.05 g/L CaCl2·2H2O,0.02 g/L FeCl3·2H2O,0.5 g/L MgSO4·7H2O,此条件下摇瓶培养甘露醇产量为61.7 g/L。进行30 L发酵罐扩大培养,根据发酵曲线得知72 h时甘露醇最大产量为80.3 g/L。

A strain which can use glucose to produce mannitol was isolated from sugarcane juice and identified as Candida parapsilosis. The production of mannitol under different fermentation conditions was studied in the paper.The recipes of optimal media was determined as 200 g / L glucose,30 g / L yeast extract,0. 05 g / L CaCl2·2H2O,0. 02 g / L FeCl3·2H2O and 0. 5 g / L MgSO4·7H2O based on the single factor test and orthogonal experiments. Under optimal conditions,the production of mannitol was 61. 7 g / L in flask culture. The fermentation enlargement was performed using strain C. parapsilosis in 30-liter fermentor. Mannitol reached the highest production of 80. 3 g / L at72 h according to the curve of fermentation.