以苹果渣为试材,用白腐菌(Trametes versicolor 500202)预处理后,分别以纤维素酶、里氏木霉和黑曲霉组合菌(Trichoderma reesei RUT-C30和Aspergillus niger UA8)糖化,半固态发酵,测定不同预处理时间下的还原糖含量、糖化产糖量和乙醇生成量。研究结果表明:(1)纤维素酶糖化和糖化菌糖化的还原糖产量均随预处理天数的延长持续增加,2种糖化方法对预处理15 d果渣的糖化产糖量分别达到200.22 g/kg、167.38 g/kg;总还原糖含量分别达到318.87 g/kg、285.07 g/kg。(2)糖化菌法对果渣重复糖化3次,还原糖总量达到389.09 g/kg。(3)纤维素酶糖化与糖化菌糖化处理果渣的乙醇产量也随着预处理天数的延长而增加,预处理15 d的果渣乙醇产量分别为201.21 mL/kg、180.66 mL/kg。2种糖化方法下,还原糖含量与乙醇生成量之间均呈极显著正相关(P<0.01)。

Apple pomace provided by Meixian branch of Hengxing Fruit Juice Co. Ltd was researched herein.Apple pomace pretreated with Trametes versicolor 500202 for different days were respectively saccharificated by cellulase,compound fungi of Trichoderma reesei RUT-C30,and Aspergillus niger UA8,and then fermented with semi-solid state. Yield of reducing sugar from pretreatment and saccharification and yield of ethanol from fermentation were determined as the function of pretreatment days. The result showed as following:( 1) with the prolonging of pretreatment days,yields of reducing sugar from saccharification of pomace with either celulase or compound fungi were both increased. Two measures reached a reducing sugar yield from sacchrification of 200. 22 g / kg and 167. 38 g / kg,respectively; a total reducing sugar content of 318. 87 g / kg and 285. 07 g / kg,respectively if pomace pretreated for 15 d.( 2) Pomace sacchrificated with compound fungi three cycles produced a total reducing sugar of 389. 09 g/kg.( 3)The yields of ethanol from the pomace saccharificated with either celulase or compound fungi were both increased with the prolong of pretreatment days and reached 201. 21 mL / kg and 180. 66 mL / kg respectively if pomace was pretreated for 15 d. With either of the two saccharification measures,total reducing sugar content and the ethanol yield from the pomace both showed a significant positive correlation( P < 0. 01).