采用滤纸片法测量抑菌圈。以细菌和真菌为供试菌种,对不同浓度的鼎湖鳞伞多糖进行了抑菌实验;运用化学方法分别测量分析了鼎湖鳞伞多糖的体外抗氧化活性,并用Vc和EDTA-2Na分别做了阳性对照。实验结果表明:鼎湖鳞伞多糖对革兰氏阳性菌有明显抑制作用。在多糖浓度为0.001 mg/mL时,枯草杆菌和金黄色葡萄球菌的抑菌圈直径分别为7.85 mm和7.91 mm,并且随着多糖浓度的增大,抑菌作用增强;而对革兰氏阴性菌和真菌的抑制作用不明显。鼎湖鳞伞多糖能够清除DPPH·和OH·以及鳌合Fe2+,具有体外抗氧化活性。

Filter method is used to measure the bacteriostatic circle. With bacteria and fungi as test stranins,inhibition experiments using polysaccharide of different concentrations from Pholiota dinghuensis Bi were carried out.Chemical method was used to measure and analyse in vitro antioxidant activity of polysaccharide from Pholiota dinghuensis Bi,with Vc and EDTA-2Na as positive control respectively. The results showed that polysaccharide from Pholiota dinghuensis Bi has obviously inhibitory effect on gram-positive bacteria. The diameter of antibacterial circle for Bacillus subtilis and Staphylococcus aureus were 7. 85 mg / mL and 7. 91 mg / mL respectively. And with the increasing concentration of polysaccharides,the inhibitory effect was stronger. However,it was not obvious for inhibition of gram-negative bacteria and fungi. Polysaccharide from Pholiota dinghuensis Bi could remove DPPH·free radicals and chelate ferrous ion( Fe2 +) and remove hydroxyl radicals and had certain of in vitro antioxidant effect.