在黑脉羊肚菌液体发酵培养基中添加不同浓度的α-萘乙酸(NAA),考察NAA对黑脉羊肚菌生物量及胞内多糖含量的影响,并对多糖的抗氧化性能进行了研究。结果表明:适当浓度的NAA能够提高黑脉羊肚菌生物量及胞内多糖含量,在其浓度为2.0μg/mL时,菌丝体干重和每克干菌丝体胞内多糖含量达到最大,分别为9.873 g/L和429.5 mg,与不添加NAA相比,分别增加了34.89%和15.68%;添加适当浓度的NAA也能提高胞内多糖对DPPH自由基的清除作用,但是降低了其对羟基自由基的清除作用。

The effects of different concentration of NAA on biomass and mycelia polysaccharide content and the antioxidant activity of mycelia polysaccharide of Morchella angusticeps Peck were studied in fermentation medium. The results showed that appropriate concentration of NAA could increase the biomass and mycelia polysaccharide content.The treatment of fermentation medium with 2. 0μg / mL of NAA could significantly increase the hyphae dry weight and mycelia polysaccharide content per gram,wherein the maximum respectively was 9. 873g / L and 429. 5mg,respectively increased 34. 89% and 15. 68% comparing to no NAA. Appropriate concentration of NAA could also improve the scavenging action of mycelia polysaccharide to DPPH,while it decreased the scavenging action of mycelia polysaccharide to hydroxyl radical.