以溶解度、抑菌圈大小及最小抑菌浓度作为评价指标,研究制备纳他霉素-大豆分离蛋白复合物的方法,以及复合对纳他霉素的增溶和抑菌效果的影响。结果表明:在纳他霉素反应浓度0.5%,纳他霉素与大豆蛋白配比4∶1,反应pH为11.5,反应时间2 h,反应温度40℃条件下纳他霉素与大豆分离蛋白复合后溶解度提高16倍,抑菌效果明显好于纳他霉素的抑菌效果,抑菌圈显著增大,对啤酒酵母、青霉、根霉、黑曲霉的最低抑菌浓度分别是2、2、4和2 mg/L,是纳他霉素单体最低抑菌浓度的50%。

In this article,the solubility,size of inhibition zone and minimum inhibitory concentration( MIC) were used as the evaluation index to study the preparation and antifungal activity of natamycin-SPI protein complex. The conditions were set as follows: natamycin concentration was 0. 5%,natamycin∶ SPI = 4∶ 1,pH = 11. 5,reaction time was 2 hours,reaction temperature was 40 ℃. The results showed that solubility of natamycin was increased by 16 times,the size of inhibition zone of natamycin-SPI protein complex was increased significantly,and the minimum inhibitory concentration to Saccharomyces cerevisiae Hansen,Penicitlium s. p,Rhizopus,Aspergilla spare were 2 mg / L,2 mg / L,4 mg / L and 2 mg / L respectively,which were half of the natamycin monomer's MIC.