为更好地研究麦芽来源的原花青素性质,建立了麦芽中原花青素的提取方法,用AB-8大孔吸附树脂对提取液分离纯化,进行动态吸附解吸实验,得到以乙醇为萃取剂的有机溶剂萃取佳条件为:乙醇体积分数60%,提取温度85℃(乙醇处于沸腾状态),料液比为1∶25(g∶mL),提取时间1.5 h。AB-8大孔吸附树脂动态吸附条件:样品质量浓度0.57 mg/mL,上样流速0.5 mL/min。动态解吸条件:洗脱剂乙醇体积分数60%,洗脱流速为1 mL/min,洗脱剂体积为65 mL,麦芽原花青素回收率可达95.2%,干基含量(纯度)为67.58%。

In order to investigate the properties of procyanidins from malt,extraction and purification methods of procyanidins were established.The crude procyanidins from malt were purified by AB-8 macroreticular resin,and the purification conditions were researched through adsorption and desorption experiment.The optimum conditions of organic solvents extraction were as follows: 60% ethanol( v/v) as the solvent,extraction temperature 85 ℃,the ratio of solvent to material at 25∶1( g∶mL),extraction time 1.5 h.The most suitable adsorption conditions were: sample concentration 0.57 mg/mL,and flow velocity 0.5 mL/min.And the desorption conditions were: 60% ethanol(v/v),flow rate 1 mL/min,elution volume 65 mL.The recovery efficiency can be reached to 95.2 %,and the purity was 67.58%.