介绍了一种酿酒酵母多倍体系列菌株的构建和快速筛选的方法,即利用HO基因表达使酿酒酵母菌株交配型发生转变,通过相应菌株间的杂交构建酿酒酵母多倍体系列菌株,与已知交配型的酿酒酵母菌株杂交,显微镜观察杂合子,快速筛选得到多倍体菌株。PCR分析及DNA含量测定结果进一步表明得到的多倍体菌株是正确的。研究所采用的构建多倍体及快速筛选的方法快捷、可靠。

This research involved an approach for construction and rapid selection of Saccharomyces cerevisiae polyploidy series strains via mating-type interconversion induced by the expression of HO gene and adequate cross. By crossing with specific mating-type strains and checking zygotes with an optical microscope,S. cerevisiae polyploidy series strains were conveniently screened. The results of PCR and Flow cytometry analysis further indicated that the polyploidy strains constructed in this research were accordance with the anticapation. Therefore,the method for construction and rapid selection of polyploidy series strains in this study was quickly and reliable.