在构建产酒酵母菌和降解苹果酸酵母菌单倍体细胞亲本库的基础上,首先优化了亲本酿酒酵母和解苹果酸裂殖酵母原生质体制备、再生和灭活条件,通过2轮基因组重组,经苹果酸降解指示培养基初筛和毛葡萄汁发酵复筛,选育获得了1株遗传稳定的融合菌株F8,其毛葡萄汁发酵液中平均总酸量仅为4.89 g/L,平均乙醇体积分数达10.39%,发酵性状接近于亲本酿酒酵母和解苹果酸裂殖酵母。实验成功证明了基因组重组技术在葡萄酒酵母选育中的有效性和高效性,同时也为毛葡萄酒酿造中一步实现产酒和降酸的新工艺奠定了基础。

In this study,the preparation,regeneration and inactivated condition of yeast protoplast were optimized on the basis of constructing a haploid cells parental library of wine yeast and malic acid degradation yeast. Then,a fusant,named F8,was isolated by preliminary screening of malic acid degradation indicator plates and second screening of grape juice fermentation through two rounds genome shuffling. The total acid and alcoholicity of grape juice fermentation liquor were 4. 89 g / L and 10. 39%( v / v),which were similar to that of parental strain. This study successfully proved that the effective and efficient of genome shuffling in the wine yeast breeding,and laid the foundation for the wine production that brewing wine and reducing acid synchronously.