以鸭血浆蛋白为原料,研究亚临界水技术不同温度(120℃、140℃、160℃、180℃)处理不同时间(0min、5 min、10 min、20 min、40 min)对其溶解性、游离氨基含量及抗氧化活性的影响。结果表明,120℃处理不同时间,蛋白的溶解性及游离氨基含量没有显著性差异;高于120℃时,随着处理温度的升高或处理时间的延长,蛋白的溶解性均降低;140℃40 min时,游离氨基含量稍有下降;160~180℃范围内,随着温度升高或处理时间延长,含量随之升高。原血浆蛋白的抗氧化能力较弱,但随着亚临界水处理温度的升高或处理时间的延长,其抗氧化活性显著提高,180℃40 min时,其DPPH·清除能力增加77.08%,还原能力增加近39倍,ABTS+清除能力增加67.65%。

Duck blood plasma was treated with subcritical water under the range of temperature from 120 ℃ to 180 ℃ and the time from 0 min to 40 min. The solubility,free amino groups content and antioxidant activity of proteins were evaluated. The results showed that there were no significant differences in the solubility and free amino groups content of proteins among the samples treated with different time at 120 ℃. As the temperature was over 120 ℃,the solubility of protein decreased with the enhancement of temperature or heating time. Free amino group content deceased at 140 ℃ for 40 min. While the temperature further increased,it would increase. Antioxidant activity of original plasma protein was low,but increased apparently as the temperature or heating time increased. The DPPH radical-scavenging activity increased by 77. 08%,the reducing power increased by 39 times than original one, and the ABTS radical scavenging activity increased by 67. 65% at 180℃ for 40 min.